Ex Vivo

Instant Digital Pathology

Conventional histology is a time-consuming and resource-intensive procedure and is characterised by long waiting times.
By using the VivaScope 2500M-G4, the excised tissue can be examined without time-consuming preparation. It allows for a rapid assessment of the removed tissue whilst still in the operating room.

VivaScope 2500M-G4

Confocal Laser Scanning Microscope

The VivaScope 2500M-G4 is a confocal laser scanning microscope designed specifically for the analysis of diagnostic biopsies and the assessment of tumour margins during surgery. Tissue can be examined immediately after an excision without  lengthy procedures. This allows for the direct assessment of tissue right in the operating room.

Tissue preparation and staining take less than a minute: for instance, a tissue sample of 1 cm x 1 cm can be stained, scanned and displayed in less than 4 minutes.

Assessment of tumour margins in conventional histology is typically a long process that requires specialised technicians, adequate facilities to perform histological sections, equipment, and material. Waiting for results often means that valuable time is lost.

The VivaScope 2500M-G4 offers an innovative alternative that saves time, money and material.

Minimal preparation
No fixation and merely a very fast staining procedure (less than a minute) are required to subsequently examine the tissue sample.
Direct Assessment
Direct assessment of excised tissue in horizontally precisely defined optical cross-sections is possible immediately after the excision. The images of excised tissue show cellular morphology, similar to conventional histology.
Considerable Time Savings
Compared to the conventional cryosections, the time required to assess the excised tissue can be drastically reduced.
Tissue Integrity
The examined tissue is not damaged in the course of the procedure and can therefore additionally be used for histological purposes.

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Technology

For the ex vivo examination of excised tissue during surgery, two lasers are used with wavelengths of 785 nm (infrared) and 488 nm (blue).
To generate reflection signals, a laser beam in the near infrared range is directed through a lens system including a beam splitter and onto the tissue to be examined. The laser beam returns through the beam splitter and hits the detector. The light source, the illuminated spot on the tissue and the aperture opening of the detector are on optically conjugated focal planes - they are confocally interconnected.

In addition, a fluorescence dye is used to highlight the DNA. The signals for the detection of the fluorescence are generated with a 488 nm laser. The fluorescence signal is used to show the individual cell nuclei of the tissue sample.

Both the reflection and fluorescence signals are detected simultaneously and combined in real time. An integrated algorithm translates the signals into H&E-like pseudocolored images without changing the image information. The resulting image contains information similar to conventional histology and can be displayed at the desired magnification.
Seminars and Training
MAVIG offers, in collaboration with Competence Centres, a well-designed and comprehensive training programme about confocal laser scanning microscopy and how to use the VivaScope products to their users.
MAVIG GmbH
VivaScope
Stahlgruberring 5
81829 Munich
Germany
Phone:
+49 (0) 89 420 96 - 280
Fax:
+49 (0) 89 420 96 - 281
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